Online Biochemical Engineering Test - Biochemical Engineering Test 2

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Total number of questions: 20.
Time allotted: 30 minutes.
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Which graphical method is used to determine an enzyme degree of cooperativity?

Hill plot

Koshland curve

Michaelis-Menten hyperbola

Can not be determined

[#]

The effect of non-competitive inhibition on a Lineweaver-Burk Plot is that

it can move the entire curve to the right

it can change the y-intercept

it can change the x-intercept

all of these

[#]

Which is not the method for producing immobilized enzymes with multifunctional reagents?

Enzymes are adsorbed on the surface active support followed by intermolecular cross linking

Functional groups are introduced on the support to react co-valently with enzymes

Enzymes are cross linked intermolecularly

None of the above

[#]

Commonly employed water insoluble supports for the covalent attachment of enzyme include

acrylamide based polymers

polypeptides

dextran

all of these

[#]

Discoloration can be achieved by using

sulphloydryl oxidase

proteases

anthocyanase

all of these

[#]

The growth of an organism on glucose is described by the following Monod model parameters: μ_m = 0.5 h^-1 and Ks =0.1 g.l^-1, if the concentration of glucose in the feed is 10 g.l^-1 and the dilution rate is set to 0.4 h^-1, then the steady state concentration of glucose in the effluent will be

0 g.l^-1

0.5 g.l^-1

1.0 g.l^-1

10 g.l^-1

[#]

Continuous culture has an advantage over to a batch cultures as

continuous reactors do not need to be shut down and cleaned as regularly as a batch reactor

most downstream and upstream processes are continuous in nature and thus the operation of continuous bioreactor can be better integrated into the overall manufacturing process

cells can be maintained at a constant physiological state simply by setting the dilution rate

all of the above

[#]

Which of the following is an example of a mathematical model?

Volume of cuboid = length x breadth x height

The Monod Model

The Michaelis Menten Equation

All of the above

[#]

A mixed fermentation is one, which produces

both alcohol and carbon dioxide

both acid and carbon dioxide

both acid and alcohol

several different kinds of acid

[#]

10.

Radial flow impellers

provide high shear conditions for breaking up bubbles

direct flow towards the walls of the reactor

Both (a) and (b)

provide low shear conditions and are suited for use in shear sensitive procesess

[#]

11.

Which of the following is not classified as an axial flow impeller?

A propeller

A marine impeller

A Rushton turbine

A hydrofoil impeller

[#]

12.

The maximum solubility of the alkane in the medium is 5 ppm. If the actual dissolved concentration of the alkane in the medium is 1 ppm. what conclusion may be drawn?

The growth rate is limited by the rate of mass transfer of the alkane into the fermentation medium

The growth rate is limited by the intrinsic kinetic properties of the cells and not by mass transfer

The growth rate is neither mass transfer nor kinetically limited

No conclusion on the growth rate can be withdrawn

[#]

13.

Pure oxygen, if used instead of air will increase oxygen transfer rates because

the saturation concentration of oxygen is higher

the bubble size is smaller

the oxygen transfer coefficient is larger

the partial pressure of oxygen in the gas phase is lower

[#]

14.

Which of the following impellers generate a flow parallel to the axis of shaft?

Axial flow

Radial flow

Both (a)and(b)

None of these

[#]

15.

Bioreactors, where vortex is formed, another dimensionless number, __________ is useful.

Stanton

Rayleigh

Bond

Froude

[#]

16.

A viscoelastic fluid is one in which the viscosity

is constant regardless of the stirrer speed or mixing time

changes during mixing but returns to its original state after mixing

increases with increasing stirrer speed

decreases with increasing stirrer speed

[#]

17.

The radius of the filter with a depth of 14 cm required for a volumetric airflow rate of 10 m³/min is approximately

1.6 m

0.6 m

6 m

[#]

18.

Maintenance cost varies from

0-4% of the capital cost

12-16% of the capital cost

4-8% of the capital cost

8-12% of the capital cost

[#]

19.

Start up expenses in a fermentation industry varies from

0-5% of the capital cost

10-15% of the capital cost

5-10% of the capital cost

15-20% of the capital cost

[#]

20.

The activated sludge process is sometime referred as

fluid bed biological oxidation system

fixed bed biological oxidation system

turning bed biological oxidation system

none of the above

[#]

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Online Biochemical Engineering Test - Biochemical Engineering Test 2

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