Biotechnology - Recombinant DNA - Discussion
Discussion Forum : Recombinant DNA - Section 1 (Q.No. 1)
1.
A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
Discussion:
25 comments Page 1 of 3.
Kalpana sharma said:
1 decade ago
The polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.
Afshan said:
1 decade ago
PCR is used for amplyfing DNA, it can be done using single or two or random primers depending on particular requirement.
JEAN BOSCO nkurikiyumukiza said:
1 decade ago
The PCR is used to magnify/amplify a target DNA. See Forensic applications.
Keshav Bhardwaj said:
1 decade ago
In the PCR techniques when we are making a rxn mix we are adding primers they helps to amplify the our DNA sequence by generating the nucleotides and adding those at a specific position, thats why we are getting the millions of copies of DNA by these oligonucleotide primer sequences.
Ankita Banerjee said:
1 decade ago
PCR is used to amplify a specific region of a DNA strand. Its amplify DNA fragments of between 0.1 and 10 kilo base pairs (kb), although some techniques allow for amplification of fragments up to 40 kb in size.
Kavita sharma said:
1 decade ago
PCR is worked like photostat machine and generate millions DNA xerox in some hours. This method is developed by Kary Mullis in 1985.
Sbr said:
1 decade ago
By utilising specific primers for desired gene we can amplify the gene of interest in vitro by using a technique called polymerase chain reaction in which we provide all the necessary components like pcr buffer, dna polymerase enzyme, primers and final and most important factor temperature.
Veena said:
1 decade ago
DNA polymerases can only extend a sequence(that is primer.)they cannot start the process of replication.They can add nucleotides at 3' end of growing strand.This is why primers are used.
Arushi sharma said:
1 decade ago
PCR is used to amplify dna segment. It has many application in our life so also known as people choice reaction.
Aarushi sharma said:
1 decade ago
This method is developed by kary mullis. It is used to amplify a particular DNA segment. It involves denaturation, annealing.
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