Biotechnology - Recombinant DNA - Discussion

Discussion :: Recombinant DNA - Section 1 (Q.No.1)

1. 

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

[A]. southern blotting
[B]. northern blotting
[C]. polymerase chain reaction
[D]. DNA replication

Answer: Option C

Explanation:

No answer description available for this question.

Kalpana Sharma said: (Aug 18, 2012)  
The polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

Afshan said: (Sep 29, 2012)  
PCR is used for amplyfing DNA, it can be done using single or two or random primers depending on particular requirement.

Jean Bosco Nkurikiyumukiza said: (Oct 17, 2012)  
The PCR is used to magnify/amplify a target DNA. See Forensic applications.

Keshav Bhardwaj said: (Nov 22, 2012)  
In the PCR techniques when we are making a rxn mix we are adding primers they helps to amplify the our DNA sequence by generating the nucleotides and adding those at a specific position, thats why we are getting the millions of copies of DNA by these oligonucleotide primer sequences.

Ankita Banerjee said: (Jul 14, 2013)  
PCR is used to amplify a specific region of a DNA strand. Its amplify DNA fragments of between 0.1 and 10 kilo base pairs (kb), although some techniques allow for amplification of fragments up to 40 kb in size.

Kavita Sharma said: (Sep 1, 2013)  
PCR is worked like photostat machine and generate millions DNA xerox in some hours. This method is developed by Kary Mullis in 1985.

Sbr said: (Apr 15, 2014)  
By utilising specific primers for desired gene we can amplify the gene of interest in vitro by using a technique called polymerase chain reaction in which we provide all the necessary components like pcr buffer, dna polymerase enzyme, primers and final and most important factor temperature.

Veena said: (Apr 22, 2014)  
DNA polymerases can only extend a sequence(that is primer.)they cannot start the process of replication.They can add nucleotides at 3' end of growing strand.This is why primers are used.

Arushi Sharma said: (Apr 26, 2014)  
PCR is used to amplify dna segment. It has many application in our life so also known as people choice reaction.

Aarushi Sharma said: (Apr 26, 2014)  
This method is developed by kary mullis. It is used to amplify a particular DNA segment. It involves denaturation, annealing.

Shalu Meena said: (Jun 2, 2014)  
PCR is used for amplifying DNA, it can be done using single or double or zig zag primers depends on particular requirement.

Vandas said: (Aug 15, 2014)  
it is a modern method in recombinant DNA technology which amplifies a short standardized region of genome(double helix DNA)through the process of DENATURING, ANNEALING AND EXTENSION.

Anjali said: (Apr 22, 2015)  
Polymerase chain reaction is a technique in molecular biology which helps in producing millions of copies of a specific DNA sequence of interest. It involves three stages:

Denaturation.
Annealing.
Extension of primers.

Tarun said: (May 8, 2015)  
PCR used in research lab for DNA cloning for sequencing, DNA based phylogeny, the diagnosis of hereditary diseases, detection of infectious diseases, paternity testing, identification of genetic fingerprints.

Rabiulbtge said: (Jul 12, 2015)  
PCR means Polymerase Chain Reaction. It first proposed by Kary Mulis in 1985. By the use of polymerase enzyme, promer, dNTPs, specific type of DNA sequence amplified to the optimum temperature.

Abdul Hadi said: (Dec 20, 2016)  
PCR is used in the different laboratories used to make many copies of a particular region of DNA.

Trishl Oza said: (Feb 6, 2017)  
PCR is used to amplify a DNA fragment.

Divya Negi said: (Mar 16, 2017)  
PCR is a process in which we amplified the DNA of an organism three steps. In this whole process, we need bacteria thermo Aquatics.

Atq said: (Mar 31, 2017)  
PCR means POLYMER CHAIN REACTION.

Dr. Mate said: (Oct 8, 2018)  
Molecules into many pecies in semiconservative manner.

Ishu said: (May 23, 2019)  
PCR means polymerase chain reaction. It is first proposed by Cary mulis in 1985. Polymerase chain reaction is biochemical technology. It is a single or few copies of DNA segment.

Swathi said: (Sep 12, 2019)  
PCR -Polymerase Chain Reaction which is used to make multiple copies of DNA.

Lilprezy said: (Oct 14, 2019)  
PCR - polymerase Chain Reaction is a technique for the rapid production of DNA fragments.

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