Biotechnology - Recombinant DNA - Discussion

Discussion Forum : Recombinant DNA - Section 1 (Q.No. 1)
A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
southern blotting
northern blotting
polymerase chain reaction
DNA replication
Answer: Option
No answer description is available. Let's discuss.
25 comments Page 2 of 3.

Rabiulbtge said:   9 years ago
PCR means Polymerase Chain Reaction. It first proposed by Kary Mulis in 1985. By the use of polymerase enzyme, promer, dNTPs, specific type of DNA sequence amplified to the optimum temperature.

Tarun said:   9 years ago
PCR used in research lab for DNA cloning for sequencing, DNA based phylogeny, the diagnosis of hereditary diseases, detection of infectious diseases, paternity testing, identification of genetic fingerprints.

Anjali said:   9 years ago
Polymerase chain reaction is a technique in molecular biology which helps in producing millions of copies of a specific DNA sequence of interest. It involves three stages:

Extension of primers.

Vandas said:   10 years ago
it is a modern method in recombinant DNA technology which amplifies a short standardized region of genome(double helix DNA)through the process of DENATURING, ANNEALING AND EXTENSION.

Shalu meena said:   1 decade ago
PCR is used for amplifying DNA, it can be done using single or double or zig zag primers depends on particular requirement.

Aarushi sharma said:   1 decade ago
This method is developed by kary mullis. It is used to amplify a particular DNA segment. It involves denaturation, annealing.

Arushi sharma said:   1 decade ago
PCR is used to amplify dna segment. It has many application in our life so also known as people choice reaction.

Veena said:   1 decade ago
DNA polymerases can only extend a sequence(that is primer.)they cannot start the process of replication.They can add nucleotides at 3' end of growing strand.This is why primers are used.

Sbr said:   1 decade ago
By utilising specific primers for desired gene we can amplify the gene of interest in vitro by using a technique called polymerase chain reaction in which we provide all the necessary components like pcr buffer, dna polymerase enzyme, primers and final and most important factor temperature.

Kavita sharma said:   1 decade ago
PCR is worked like photostat machine and generate millions DNA xerox in some hours. This method is developed by Kary Mullis in 1985.

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