Biotechnology - Recombinant DNA - Discussion

Discussion Forum : Recombinant DNA - Section 1 (Q.No. 1)
1.
A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
southern blotting
northern blotting
polymerase chain reaction
DNA replication
Answer: Option
Explanation:
No answer description is available. Let's discuss.
Discussion:
25 comments Page 1 of 3.

Sbr said:   1 decade ago
By utilising specific primers for desired gene we can amplify the gene of interest in vitro by using a technique called polymerase chain reaction in which we provide all the necessary components like pcr buffer, dna polymerase enzyme, primers and final and most important factor temperature.

Keshav Bhardwaj said:   1 decade ago
In the PCR techniques when we are making a rxn mix we are adding primers they helps to amplify the our DNA sequence by generating the nucleotides and adding those at a specific position, thats why we are getting the millions of copies of DNA by these oligonucleotide primer sequences.

Kalpana sharma said:   1 decade ago
The polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence.

Anjali said:   9 years ago
Polymerase chain reaction is a technique in molecular biology which helps in producing millions of copies of a specific DNA sequence of interest. It involves three stages:

Denaturation.
Annealing.
Extension of primers.

Ankita Banerjee said:   1 decade ago
PCR is used to amplify a specific region of a DNA strand. Its amplify DNA fragments of between 0.1 and 10 kilo base pairs (kb), although some techniques allow for amplification of fragments up to 40 kb in size.

Tarun said:   9 years ago
PCR used in research lab for DNA cloning for sequencing, DNA based phylogeny, the diagnosis of hereditary diseases, detection of infectious diseases, paternity testing, identification of genetic fingerprints.

Rabiulbtge said:   9 years ago
PCR means Polymerase Chain Reaction. It first proposed by Kary Mulis in 1985. By the use of polymerase enzyme, promer, dNTPs, specific type of DNA sequence amplified to the optimum temperature.

Veena said:   1 decade ago
DNA polymerases can only extend a sequence(that is primer.)they cannot start the process of replication.They can add nucleotides at 3' end of growing strand.This is why primers are used.

Vandas said:   10 years ago
it is a modern method in recombinant DNA technology which amplifies a short standardized region of genome(double helix DNA)through the process of DENATURING, ANNEALING AND EXTENSION.

Ishu said:   5 years ago
PCR means polymerase chain reaction. It is first proposed by Cary mulis in 1985. Polymerase chain reaction is biochemical technology. It is a single or few copies of DNA segment.


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