Biotechnology - Recombinant DNA - Discussion

Discussion Forum : Recombinant DNA - Section 1 (Q.No. 1)
1.
A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
southern blotting
northern blotting
polymerase chain reaction
DNA replication
Answer: Option
Explanation:
No answer description is available. Let's discuss.
Discussion:
25 comments Page 2 of 3.

Shalu meena said:   1 decade ago
PCR is used for amplifying DNA, it can be done using single or double or zig zag primers depends on particular requirement.

Vandas said:   10 years ago
it is a modern method in recombinant DNA technology which amplifies a short standardized region of genome(double helix DNA)through the process of DENATURING, ANNEALING AND EXTENSION.

Anjali said:   9 years ago
Polymerase chain reaction is a technique in molecular biology which helps in producing millions of copies of a specific DNA sequence of interest. It involves three stages:

Denaturation.
Annealing.
Extension of primers.

Tarun said:   9 years ago
PCR used in research lab for DNA cloning for sequencing, DNA based phylogeny, the diagnosis of hereditary diseases, detection of infectious diseases, paternity testing, identification of genetic fingerprints.

Rabiulbtge said:   9 years ago
PCR means Polymerase Chain Reaction. It first proposed by Kary Mulis in 1985. By the use of polymerase enzyme, promer, dNTPs, specific type of DNA sequence amplified to the optimum temperature.

Abdul hadi said:   8 years ago
PCR is used in the different laboratories used to make many copies of a particular region of DNA.

Trishl oza said:   7 years ago
PCR is used to amplify a DNA fragment.

Divya negi said:   7 years ago
PCR is a process in which we amplified the DNA of an organism three steps. In this whole process, we need bacteria thermo Aquatics.
(1)

Atq said:   7 years ago
PCR means POLYMER CHAIN REACTION.

Dr. Mate said:   6 years ago
Molecules into many pecies in semiconservative manner.


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