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Biochemistry - Protein Purification

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Exercise : Protein Purification - Section 1
  • Protein Purification - Section 1
1.
During successful purification scheme, this may be expected that the
specific activity increases
specific activity decreases
number of proteins in the sample decreases
both (a) and (c)
Answer: Option
Explanation:
No answer description is available. Let's discuss.
2.
In ion-exchange chromatography
proteins are separated on the basis of their net charge
proteins are separated on the basis of their size
proteins are separated on the basis of their shape
either (b) or (c)
Answer: Option
Explanation:
No answer description is available. Let's discuss.
3.
Which of the following may be added to stabilize the protein after yeast cells disruption?
NaCl
Protease inhibitor
AMP
All of these
Answer: Option
Explanation:
No answer description is available. Let's discuss.
4.
Gel-filtration chromatography separates on the basis of
size and shape using porous beads packed in a column
size using porous beads packed in a column
shape using porous beads packed in a column
none of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
5.
Affinity chromatography deals with the
specific binding of a protein constituents for another molecule
protein - protein interaction
protein - carbohydrate interaction
none of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
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