Biochemistry - Protein Purification - Discussion

Gel permeation chromatography is known as size exclusion chromatography. And purifies the protein by moving through the pores in the beads, bigger size of peptides will move between the beads or will move through the coners as there size is more then the pore size of the beads. And small moleculez move through the pores in the beads and hence takes longer time to elute out, hence smaller the molecule slower the molecule. And shape also plays a very vital role here, if the shape is in such a way that the frictional force on it is less then it moves faster and if more then slower. And the answer should be A.

Gel filtration chromatography is also known as size exclusion chromatography. The best option here is B because it is used to separate size using porous bead packed in a column.