Biochemistry - Protein Purification - Discussion

Discussion Forum : Protein Purification - Section 1 (Q.No. 1)
1.
During successful purification scheme, this may be expected that the
specific activity increases
specific activity decreases
number of proteins in the sample decreases
both (a) and (c)
Answer: Option
Explanation:
No answer description is available. Let's discuss.
Discussion:
3 comments Page 1 of 1.

Susheel said:   1 year ago
Both A and C are correct.

Since, specific activity is inversely proportional to the total number of proteins.

Najmoddin said:   3 years ago
Both A and C are correct.

Since specific activity is inversely proportional to the total number of protein.

Therefore as the number of protein decreases, the specific activity increases.

Harshal said:   7 years ago
No.of proteins should decrease. then only we will be left with the protein of interest.

Specific activity = protein activity/ total mg of protein present.

So as we proceed towards more purification steps, we see increase in specific activity.

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