Biochemistry - Protein Purification - Discussion

Discussion :: Protein Purification - Section 1 (Q.No.1)


During successful purification scheme, this may be expected that the

[A]. specific activity increases
[B]. specific activity decreases
[C]. number of proteins in the sample decreases
[D]. both (a) and (c)

Answer: Option D


No answer description available for this question.

Harshal said: (Mar 10, 2016)  
No.of proteins should decrease. then only we will be left with the protein of interest.

Specific activity = protein activity/ total mg of protein present.

So as we proceed towards more purification steps, we see increase in specific activity.

Najmoddin said: (Oct 6, 2019)  
Both A and C are correct.

Since specific activity is inversely proportional to the total number of protein.

Therefore as the number of protein decreases, the specific activity increases.

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