Online Biotechnology Test - Biotechnology Test - Random

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Total number of questions: 20.
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Phagemid consist of

plasmid vector carrying λ phage's cos site

plasmid vector carrying λ attachment (λ att) site

plasmid vector carrying origin of replication of λ phage only

plasmid vector carrying origin of replication of plasmid only

[#]

Maximum size of foreign DNA that can be inserted into a replacement vector is

25-30 kb

18-20 kb

20-25 kb

40-50 kb

[#]

What are the names of the two genders of haploid yeast cells?

A and Alpha

M and F

Y(a)and Y(b)

None of these

[#]

Which of the following experimental results does not support Mitchell's chemiosmosis theory?

Electron transport in isolated mitochondria was shown to result in acidification of the medium

Addition of dinitrophenol to isolated mitochondria was shown to inhibit electron transport, but had no effect on ATP synthesis

Addition of dinitrophenol to isolated mitochondria during electron transport was shown to inhibit acidification of the medium

An artificial proton gradient across the inner mitochondrial membrane drives ATP synthesis in the absence of electron transport

[#]

Which part of a plant contains the male sex cell?

Ova

Style

Stamen

Pollen

[#]

In the meiotic cell divison in daughter cells are produced by two successive division in which

first division is equational, second is reductional

first division is reductional and second is equational

both divisions are reductional

both divisions are equational

[#]

Silver thiosulphate is added to culture medium as it helps to

maintain the pH

remove toxic phenolics from plant cells

prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.

all of the above

[#]

In a plant tumour cell

complete Ti plasmid is incorporated in plant nuclear DNA

different parts of the Ti-plasmid are incorporated

only a small specific segment of callus T DNA is incorporated

may vary from plant to plant

[#]

Advantage of microprojectile method over microinjection method for gene transfer in plants include

intact cells are used

method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall

gene can be transferred to many cells simultaneously

all of the above

[#]

10.

Phosphinothricin acetyl transferase is encoded by

gene bxn in Klebsiella pneumoniae

bar gene in Streptomyces spp

both (a) and (b)

none of these

[#]

11.

Which of the following have been used to oxidize ammonia and to prevent algal growth?

growth of hypomicrobium in presence of added methanol

Micrococcus denitrificans cells encapsulated in liquid membranes

Both (a) and (b)

Nitrosomonas europaen

[#]

12.

When farmers breed new plants they choose the pollen from one variety of plant and place it onto the stigma of another variety of plant. This type of plant breeding is known as

selective breeding

pollination

seed collection

cuttings

[#]

13.

In which phase, each chromosome replicates to produce two sister chromatids?

Anaphase

Interphase

Metaphase

Prophase

[#]

14.

Which of the following is the normal cell cycle, primarily regulated at checkpoints?

Metaphase in mitosis

Anaphase, G1 boundary

G1-S boundary

S-G2 boundary

[#]

15.

In animal cell culture, particularly mammalian cell culture, transformation means

uptake of new genetic material

phenotypic modifications of cells in culture

both (a) and (b)

release of genetic information

[#]

16.

To prevent the accumulation of lactate

low glutamine concentration is required

high glutamine concentration is required

low glucose concentration is required

high glucose concentration is required

[#]

17.

In case of Baculoviruses, the foreign gene

is inserted downstream of polyhedrin promoter

is inserted downstream of viral origin of replication

is inserted between the polyhedrin promoter and downstream sequences

is inserted randomly in viral genome

[#]

18.

Which of the following is the process of choosing parent organisms for the characteristics that is wanted in their offspring?

Active selection

Reproductive selection

Selective breeding

Breeding selection

[#]

19.

In which year, Dolly the first mammalian clone was born?

1995

1997

1996

1998

[#]

20.

Ales are the beer in which fermentation is carried out using

top yeast

bottom yeast

either of the above

middle yeast

[#]

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