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Biotechnology - Cloning Vectors

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Exercise : Cloning Vectors - Section 1
  • Cloning Vectors - Section 1
1.
Maximum size of foreign DNA that can be inserted into a replacement vector is
25-30 kb
18-20 kb
20-25 kb
40-50 kb
Answer: Option
Explanation:
No answer description is available. Let's discuss.
2.
Which of the following is not true about phagemid?
Contain functional origin of replication of the plasmid and λ phage
May be propagated as a plasmid or as phage in appropriate strain
Contain λ att site
Can only be propagated as phage
Answer: Option
Explanation:
No answer description is available. Let's discuss.
3.
pBR 322 has/have which of the following selection marker(s)?
Ampr
Tetr
Both (a) and (b)
Kanr
Answer: Option
Explanation:
No answer description is available. Let's discuss.
4.
A plasmid can be considered as a suitable cloning vector if
it can be readily isolated from the cells
it possesses a single restriction site for one or more restriction enzymes
insertion of foreign DNA does not alter its replication properties
All of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
5.
Difference between λ gt 10 and λ gt 11 vectors is that
λ gt 11 is an expression vector
λ gt 10 is an expression vector
λ gt 10 is a replacement vector
λ gt 11 is a replacement vector
Answer: Option
Explanation:
No answer description is available. Let's discuss.
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