Microbiology - Polymerase Chain Reaction - Discussion
Discussion Forum : Polymerase Chain Reaction - Section 1 (Q.No. 5)
5.
The original enzyme(s) used in PCR reaction is/are known as
Discussion:
8 comments Page 1 of 1.
Soumya Ranjan Mohapatra said:
1 decade ago
E.coli polymerase can't survive high temperature so the Taq polymerase is used as it is extracted from thermophilic bacteria Thermus aquaticus.
Kencho said:
1 decade ago
Taq polymerase is used. It is heat stable enzymes extracted from the thermophilus bacteria which dwells in the hot springs and geysers.
Adarsh said:
1 decade ago
Normally taq polymerase is used because it is stable at high temperature during denaturation step. So something is wrong in question.
Josine said:
3 years ago
Exactly, it is Taq polymerase, the enzyme to be used because it is the one which is thermostable.
Gulab said:
3 years ago
Exactly, it is Taq polymerase, the enzyme to be used because it is the one which is thermostable.
Sya said:
1 decade ago
But we also use Taq polymerase to lay out the primers after denaturation.
Sandra said:
6 years ago
Yes, Taq polymerase is usually used in PCR.
Kiran jamshed said:
9 years ago
Taq polymerase used in PCR.
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