Microbiology - Polymerase Chain Reaction - Discussion

Discussion :: Polymerase Chain Reaction - Section 1 (Q.No.5)


The original enzyme(s) used in PCR reaction is/are known as

[A]. E. coli DNA polymerase
[B]. E. coli RNA polymerase
[C]. Taq polymerase
[D]. all of these

Answer: Option A


No answer description available for this question.

Sya said: (Oct 13, 2013)  
But we also use Taq polymerase to lay out the primers after denaturation.

Adarsh said: (Oct 16, 2013)  
Normally taq polymerase is used because it is stable at high temperature during denaturation step. So something is wrong in question.

Soumya Ranjan Mohapatra said: (Apr 13, 2014)  
E.coli polymerase can't survive high temperature so the Taq polymerase is used as it is extracted from thermophilic bacteria Thermus aquaticus.

Kencho said: (Aug 15, 2014)  
Taq polymerase is used. It is heat stable enzymes extracted from the thermophilus bacteria which dwells in the hot springs and geysers.

Kiran Jamshed said: (Feb 5, 2017)  
Taq polymerase used in PCR.

Sandra said: (Nov 29, 2019)  
Yes, Taq polymerase is usually used in PCR.

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