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Microbiology - Polymerase Chain Reaction

Exercise : Polymerase Chain Reaction - Section 1
  • Polymerase Chain Reaction - Section 1
1.
Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?
HIV-1 and HIV-2 viruses
Hepatitis B virus
Mycobacterium tuberculosis
All of these
Answer: Option
Explanation:
No answer description is available. Let's discuss.
2.
Polymerase chain reaction basically consists of
two steps
three steps
four steps
five steps
Answer: Option
Explanation:
No answer description is available. Let's discuss.
3.
The PCR/sequencing approach to rRNA is most likely to give information about
the species to which the bacteria are most closely related
morphology of the bacterium
the type of energy metabolism the bacterium has
whether the bacterium is motile
Answer: Option
Explanation:
No answer description is available. Let's discuss.
4.
The efficiency of amplification in PCR in later cycles is reduced due to
reduction in substrate concentration
insufficient enzyme and time to synthesize mass quantity of DNA
build up of PCR product which competes with primers for hybrid formation
all of these
Answer: Option
Explanation:
No answer description is available. Let's discuss.
5.
The original enzyme(s) used in PCR reaction is/are known as
E. coli DNA polymerase
E. coli RNA polymerase
Taq polymerase
all of these
Answer: Option
Explanation:
No answer description is available. Let's discuss.
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