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Microbiology - Polymerase Chain Reaction - Discussion

Discussion Forum : Polymerase Chain Reaction - Section 1 (Q.No. 4)
Polymerase Chain Reaction
4.
The efficiency of amplification in PCR in later cycles is reduced due to
reduction in substrate concentration
insufficient enzyme and time to synthesize mass quantity of DNA
build up of PCR product which competes with primers for hybrid formation
all of these
Answer: Option
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