Microbiology - Polymerase Chain Reaction - Discussion
Discussion Forum : Polymerase Chain Reaction - Section 1 (Q.No. 2)
2.
Polymerase chain reaction basically consists of
Discussion:
5 comments Page 1 of 1.
Bijaya Baijayantee Behera said:
2 years ago
Denaturation
Annealing
Extention.
Pneumonics for this is DAE.
The first is at 90 to 97 degree.
2nd step is 68degree.
3rd step is 72 degree.
Annealing
Extention.
Pneumonics for this is DAE.
The first is at 90 to 97 degree.
2nd step is 68degree.
3rd step is 72 degree.
(1)
Somnath said:
1 decade ago
1. Denaturation 94 C.
2. Annealing at 54 C.
3. Elongation at ca. 72 C.
2. Annealing at 54 C.
3. Elongation at ca. 72 C.
Neha Saxena said:
1 decade ago
1. Denaturation 94 - 96 C.
2. Annealing at -68 C.
3. Elongation at ca. 72 C.
2. Annealing at -68 C.
3. Elongation at ca. 72 C.
Lovely said:
1 decade ago
The first step is to denature the dna strand and the second step is to anneal the two primers to the 3' end and the third step is for dna extension.
Mahi desai said:
1 decade ago
Polymerase chain reaction basically five steps are there but you say that only three steps are their. Please explain me which are this three steps.
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