Microbiology - Identification of Bacteria - Discussion

Discussion :: Identification of Bacteria - Section 1 (Q.No.10)


The production of sufficient acid by fermentation of glucose leads to decrease in pH such that pH of the medium falls below 4.5. Which of the following test can detect it?

[A]. Indole test
[B]. Methyl red test
[C]. Citrate utilization test
[D]. Voges-Proskauer test

Answer: Option B


No answer description available for this question.

Sana Akram said: (Mar 2, 2016)  
Why is it?

Srikanth said: (Mar 2, 2020)  
These tests generally fall under the IMViC series of tests used to differentiate the bacteria.

Indole - checks for the conversion of trp to indole.

Methyl red test - the glucose is generally fermented to the lactic acid, acetic acid, and formic acid. Due to the formation of these acids, the ph of medium falls below 4.5.

Citrate - tests whether bacteria can use citrate as a sole source of carbon and energy.

VP test - detects the formation of acetoin as a positive result.

Kiran Subhashish Behera said: (May 22, 2021)  
What is the medium for the methyl red test?

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