Biotechnology - Recombinant DNA - Discussion

Correct answer: the application of high voltage pulses.

The other options describe different processes:

The process of separating charged molecules through a gel maintained in an electric field: This refers to gel electrophoresis, a technique used to separate DNA, RNA, or proteins based on their size and charge.

The process of combining foreign DNA to an electrically charged vector molecule: This could refer to a method of ligation or DNA cloning but is not specific to electroporation.

The process of multiplication of the cells: This refers to cell proliferation or cell division, which is not related to electroporation.

Option A) is the definition of electrophoresis.

Option B) is incorrect because the vector molecule cannot be charged in electroporation.

Option D) is the basic definition of cell division so the answer is definitely option C) because electroporation is applying a high voltage pulse to create a temporary pore in the plasma membrane of the cell for introducing the foreign DNA.

Electroporation is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular biology as a way of introducing some substance into a cell, such as loading it with a molecular probe, a drug that can change the cell's function, or a piece of coding DNA.

Electroporation is a mechanical method used to introduce polar molecules into a host cell through the cell membrane.

In this procedure, a large electric pulse temporarily disturbs the phospholipid bilayer, allowing molecules like DNA to pass into the cell.

Electroporation is one of the methods through which the plasma membrane of the E. Coli bacteria is made permeable by using agents like cacl, so that the plasmid can easily pass inside the cytoplasm.

I think the correct answer is that its the process by which polar molecules (DNA) is introduced into the cell through application of high voltage (which creates holes in the Plasma membrane).

Electroporation is a technique used to introduce molecules such as DNA, RNA, proteins or drugs into cells by applying brief electrical plus to create temporary pores in the cell membrane.

The correct answer is A. The definition of electrophoresis is the separation of charged molecules based on the electric field.

This techniques used to express the target protein in either plant cell or animal cell. One type of gene expression method.

Answers B according to the definition of teleportation.

The application of electric city to transfer foreign DNA.