Biotechnology - Recombinant DNA - Discussion

Discussion Forum : Recombinant DNA - Section 1 (Q.No. 7)
7.
Electroporation is
the process of separating charged molecules through a gel maintained in an electric field
the process of combining foreign DNA to an electrically charged vector molecule
the application of high voltage pulses
the process of multiplication of the cells
Answer: Option
Explanation:
No answer description is available. Let's discuss.
Discussion:
17 comments Page 1 of 2.

Faisal Khan said:   1 year ago
Correct answer: the application of high voltage pulses.

The other options describe different processes:

The process of separating charged molecules through a gel maintained in an electric field: This refers to gel electrophoresis, a technique used to separate DNA, RNA, or proteins based on their size and charge.

The process of combining foreign DNA to an electrically charged vector molecule: This could refer to a method of ligation or DNA cloning but is not specific to electroporation.

The process of multiplication of the cells: This refers to cell proliferation or cell division, which is not related to electroporation.

Sravani said:   1 year ago
Electroporation is a technique used to introduce molecules such as DNA, RNA, proteins or drugs into cells by applying brief electrical plus to create temporary pores in the cell membrane.

Sneha K said:   7 years ago
Option A) is the definition of electrophoresis.

Option B) is incorrect because the vector molecule cannot be charged in electroporation.

Option D) is the basic definition of cell division so the answer is definitely option C) because electroporation is applying a high voltage pulse to create a temporary pore in the plasma membrane of the cell for introducing the foreign DNA.

Preeti said:   8 years ago
Please tell which is the right answer? Explain.

Girma Gashu said:   8 years ago
Answers B according to the definition of teleportation.

The application of electric city to transfer foreign DNA.

Arnav said:   8 years ago
The Correct answer is C, because it separates both un-charged & charged molecules.

Siddharth said:   9 years ago
Please give me the answer.

Molen Dias said:   9 years ago
Electroporation is one of the methods through which the plasma membrane of the E. Coli bacteria is made permeable by using agents like cacl, so that the plasmid can easily pass inside the cytoplasm.

Sidra said:   9 years ago
Answer is A, according to the definition of electroporation.

AMMU said:   10 years ago
Electroporation is a mechanical method used to introduce polar molecules into a host cell through the cell membrane.

In this procedure, a large electric pulse temporarily disturbs the phospholipid bilayer, allowing molecules like DNA to pass into the cell.


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