Online Biotechnology Test - Biotechnology Test 6

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Total number of questions: 20.
Time allotted: 30 minutes.
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For gene probes to be useful they must

be large enough to contain gene-specific sequences

be labeled in some manner to allow detection

both (a) and (b)

none of the above

[#]

Which of the following vector can maintain the largest fragment of foreign DNA?

YAC

Cosmid

Plasmid

Phage

[#]

Difference between λ gt 10 and λ gt 11 vectors is that

λ gt 11 is an expression vector

λ gt 10 is an expression vector

λ gt 10 is a replacement vector

λ gt 11 is a replacement vector

[#]

Chemically synthesized DNA sequences for the two chains are separately inserted into the plasmid pBR 322 by the side of

β-Galactosidase

galactokinase

acid phosphatase

glucokinase

[#]

How many nucleotides are there in Ss RNA molecule of picorna virus causing FMD?

1000

5000

8000

10000

[#]

When guard cells swell

stomata close

stomata open

photosynthesis stops

evaporation stops

[#]

When do kinetochore microtubules pull chromosomes towards opposite spindle poles?

In anaphase A

In anaphase B

In metaphase

In telophase

[#]

The phenomenon of the reversion of mature cells to the meristematic state leading to the formation of callus is known as

redifferentiation

dedifferentiation

either (a) or (b)

none of these

[#]

Very high sugar concentration (40-100 g/l) have been used

in specialized secondary metabolite production

to adjust the osmotic potential of the media in short term treatment for regeneration

Both (a) and (b)

none of these

[#]

10.

Which is/are the naturally occurring plant auxins?

Indole acetic acid (IAA)

Naphthalenacetic acid (NAA)

2,4-dichlorophenoxyacetic acid

All of the above

[#]

11.

Which of the following is true about T DNA?

Integration of T DNA can occur at many different, apparently random, sites in the plant nuclear DNA

Integration of T DNA occurs only at one specific sites in the plant nuclear DNA

Integration of T DNA occurs at two specific sites in the plant nuclear DNA

Integration of T DNA occurs at one site that may be random in the plant nuclear DNA

[#]

12.

Establishment of embryogenic cell suspensions with high regeneration potential is a pre-requisite for successful

protoplast culture

detection of nucleic acid sequence

restriction of fragment length polymorphisms

microinjection

[#]

13.

The encapsulation of somatic embryos can be carried out by

automatic encapsulation process

gel complexation

both (a) and (b)

coating proteins

[#]

14.

Which of the following accumulates from factory waste?

Pseudomonas aeruginosa

Thiobacillus

Pseudomonas putida

Zoogloea ramigera

[#]

15.

Milbemycin is known to exhibit a broad spectrum of activity against

agricultural pests

agricultural herbs

agricultural insects

all of these

[#]

16.

At the __________ checkpoint, cell growth is controlled.

G₁

G₂

[#]

17.

What is the pH of the medium when molasses is used as substrate for the production of citric acid?

[#]

18.

Antibiotics are typically produced in fed batch reactors because

antibiotic yields are generally higher when cells enter the stationary phase

the precursors are often toxic to the cells

antibiotic yields are generally higher when cell growth slows

All of the above

[#]

19.

Which of the following event occurs during third phase of growth of P chrysogenum?

Concentration of antibiotic increases in the medium

Autolysis of the medium starts

Slight rise in pH due to liberation of ammonia

All of the above

[#]

20.

pH of the fermentation medium for chlorotetracyclins is

5-6

6-7

7-8

8-9

[#]

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Online Biotechnology Test - Biotechnology Test 6

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