Online Biotechnology Test - Biotechnology Test 1

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Total number of questions: 20.
Time allotted: 30 minutes.
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pBR 322 has/have which of the following selection marker(s)?

Amp^r

Tet^r

Both (a) and (b)

Kan^r

[#]

Single stranded vectors are useful

for sequencing of cloned DNA

for oligonucleotide directed mutagenesis

for probe preparation

all of the above

[#]

λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to

6-7 kb

1-2 kb

40-44 kb

20-23 kb

[#]

The two chains are joined together to constitute native insulin using

sodium dissulphonate and sodium sulphite

sodium dissulphonate and sodium sulphate

sodium sulphate and sodium sulphite

sodium sulphate only

[#]

A very common mutualistic, symbiotic relationship between a fungus and the roots of a plant is

lichen

mycorrhizal

ascomycete

basidiomycete

[#]

Chemical energy is supplied to eukaryotic cells by

chloroplasts and golgi complexes

mitochondria and ribosomes

mitochondria only

mitochondria and chloroplasts

[#]

Which of the following can be found as a part of the mitochondrial matrix?

Ribosomes

DNA

Enzymes

All of these

[#]

What is meant by 'Organ culture' ?

Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid

Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal

Cultivation of organs in a laboratory through the synthesis of tissues

The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body

[#]

Protoplasts are the cells devoid of

cell membrane

cell wall

both cell wall and cell membrane

none of these

[#]

10.

Neutralized activated charcoal is occasionally added to young regenerating cultures to

remove toxic phenolics produced by the stressed plant cell

help to remove plants growth regulators introduced at an earlier stage

both (a) and (b)

maintain the pH of the medium

[#]

11.

Metabolic interference is a term used to describe a method to metabolize a compound and prevent the synthesis of something that is normally produced. What compound(s) have been targeted for metabolic interference in tomato?

ACC (1-aminocyclopropane-1-carboxylic acid)

SAM (S-adenosylmethionine)

Both (a) and (b)

AOA (aminooxyacetic acid)

[#]

12.

Which of the following has been confirmed on sugarcane genotypes?

Bacilliform virus

Cytomegalovirus

Varicella-zoster virus

Erythrovirus

[#]

13.

Which of the following is the most effective for the rice sheath blight pathogen, Pellicularia sasakii?

Polyoxin D

Polyoxin B

Polyoxin L

Polyoxin C

[#]

14.

If two genes show 50% recombination

they are on different chromosomes

they are on same chromosome but far apart

both (a) and (b)

they are closely located on the same chromosome

[#]

15.

DNA replication is controlled at which of the following checkpoint?

G₁

G₂

[#]

16.

Range of osmolarity tolerated/accepted in mOsm/Kg of H₂O by mammalian cells is

150-300

280-360

300-325

360-400

[#]

17.

Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

episomally only

integrated only

either episomally or integrated at high copy number

either episomally or integrated at low copy number

[#]

18.

What are different substrates used for ethanol production?

Starch containing substrate

Juices from sugarcane or molasses or sugar beet

Waste product from wood or processed wood

All of the above

[#]

19.

Sake is

rice beer or wine of India

wheat beer or wine

barley beer or wine

rice beer or wine of Japan

[#]

20.

Penicillin is produced by

aerobic fermentation

anaerobic fermentation

aerobic fermentation followed by anaerobic fermentation

anaerobic fermentation followed by aerobic fermentation

[#]

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Online Biotechnology Test - Biotechnology Test 1

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