Online Biochemical Engineering Test - Biochemical Engineering Test 6

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Total number of questions: 20.
Time allotted: 30 minutes.
Each question carries 1 mark; there are no negative marks.
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In a Lineweaver-Burk Plot, competitive inhibitor shows which of the following effect?

It moves the entire curve to right

It moves the entire curve to left

It changes the x-intercept

It has no effect on the slope

[#]

To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as

reaction rate/rate if not slowed by diffusion

rate if not slowed by diffusion/ reaction rate

actual reaction rate/ rate if not slowed by diffusion

rate if not slowed by diffusion/ actual reaction rate

[#]

Fluidized bed bioreactors provide higher mass transfer rates than packed bed bioreactors because

mixing is higher in fluidized bed bioreactors

particles move with the fluid in a fluidized bed bioreactor

immobilized particles are smaller in the fluidized bed bioreactors

all of the above

[#]

Yield coefficient represents

total biomass or product produced

conversion efficiency of a substrate into product

conversion rate of a substrate into biomass or product

production time of biomass or product

[#]

Which of the following would not be subjected to the "glucose effect"?

Aspergillus fumigatus

Saccharomyces cerevisiae

Escherichia coli

Aspergillus niger

[#]

Toxins accumulated in a reactor can lead to low biomass yields probably due to which of the following?

NAD⁺ is diverted away from homeostasis to anabolism

ATP is diverted away from homeostasis to anabolism

NAD⁺ is diverted away from anabolism to homeostasis

ATP is diverted away from anabolism to homeostasis

[#]

In the batch-fed mode, substrate will be added

only at the beginning of the fermentation process

periodically throughout the fermentation process

only at the end of the fermentation process

substrate is never added

[#]

A strain of Escherichia coli has a maximum specific growth rate of 0.8 h^-1 on a glucose based medium. If this organism is being grown in a chemostat with a dilution rate of 1.2 h^-1, then at steady state the concentration of E. coli in the same medium will

increase

be zero

change randomly

decrease

[#]

Which of the following is best described as secondary metabolite?

Acetic acid produced from the oxidation of ethanol

Ethanol from the fermentation of glucose

Penicillin

Citric acid from the partial oxidation of glucose

[#]

10.

The contents in jet loop reactor are mixed through

compressed air

a mechanical agitator

external pumping

any of these

[#]

11.

Wash out in steady state fermentation occurs when

dilution rate is less than maximum specific growth rate

dilution rate is higher than the maximum specific growth rate

cell concentration reaches the maximum

specific growth rate is maximum

[#]

12.

The main function(s) of a draft tube in an air lift fermenter is/are to

increase the solubility of oxygen

distribute shear forces throughout the reactor and minimise bubble coalescence

concentrate shear forces around the sparger

concentrate shear forces near the disengagement zone

[#]

13.

Which of the following impellers will provide radial flow?

Paddles

Flat blade turbines

Disk flat blade turbines

All of the above

[#]

14.

To increase the oxygen transfer rate in a shake flask culture system, which of the following (s) is/are followed?

Liquid volume in the flask is reduced

Stirrer speed is increased

Baffled flasks are used

All of the above

[#]

15.

During aeration, a cylindrical reactor had a height of 10 m. Without aeration, the height was 7.5 m. Under these aeration and mixing conditions, the gas hold up in the reactor is

100%

75%

50%

25%

[#]

16.

The power required by an impeller in a gas sparged system compare to the power required by impeller operating at same speed in a gas free liquid is usually

lesser

higher

same

may be lesser or higher depending upon the geometry

[#]

17.

The mechanical means of accomplishing sterilization of fermentation media/equipment is

ultrasonic

radiation

chemical agents

none of these

[#]

18.

Analogus to the molecular diffusion, the x-directional flux of microorganisms suspended in a medium due to the axial mixing can be represented as (where J_n is flux component 'n', c_n is concentration of'n')

J_n = -dc_n/dx

J_n = dc_n/dx

J_n = -D dc_n/dx

J_n = D dc_n/dx

[#]

19.

The heat conduction in dry air is

less rapid than in steam

more rapid than in steam

similar to steam

none of these

[#]

20.

Which of the following chemical is sometime added in the process of coagulation and flocculation?

Aluminum sulphate

Aluminum oxide

Calcium chloride

None of these

[#]

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Online Biochemical Engineering Test - Biochemical Engineering Test 6

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