Online Biochemical Engineering Test - Biochemical Engineering Test 4

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Total number of questions: 20.
Time allotted: 30 minutes.
Each question carries 1 mark; there are no negative marks.
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Which of the following activity is possible by transferases?

Transfer of methyl groups

Transfer of glycosyl group

Both (a) and (b)

None of these

[#]

The active site of an enzyme differs from an antibody-antigen binding site in that the enzyme active site

contains modified amino acids

catalyzes a chemical reaction

is complementary to a specific ligand

contains amino acids without side chains

[#]

The equation for the rate of product formation for simple enzyme reaction is given by (Where r_max, maximum reaction rate, C_s substrate concentration, C_p product concentration ES, C_ES enzyme-substrate concentration)

r_p = r_max C_s/(K_m+C_s)

r_p= r_max C_ES/(K_m+ C_ES)

r_p = r_max C_s/(K_m+C_ES)

r_p = r_max C_s/(K_m+C_p)

[#]

The slope of Lineweaver Burk plot for Michaelis Menten equation is

V_max/K_m

K_m/V_max

1/K_m

K_m.V_max

[#]

Which is true about rennet?

It is a mixture of protease chymosin and pepsin

It is a mixture of rennin and pepsin

Both (a) and (b)

none of the above

[#]

The enzyme used to reduce bitterness of grapes commonly contains

α-L-rhamonosidase

β-d-glucosidase

β- galactosidase

both (a) and (b)

[#]

A culture system with constant environmental conditions maintained through continual provision of nutrient and removal of wastes is called __________ culture system.

continuous

batch

fed-batch

semi continuous

[#]

When two populations compete for a single growth limiting substrate in a continuous fermenter, which organism would not be washed out?

Organism maintaining the highest substrate concentration

Organism maintaining the lowest substrate concentration

Both (a) and (b)

Organism maintaining the moderate substrate concentration

[#]

Low dissolved oxygen concentrations leads to

low biomass yields

high biomass yields

no effect on biomass yields

none of the above

[#]

10.

The specific growth rate (μ) is defined as

the concentration of biomass in the reactor

rate of increase of total biomass in a reactor

the rate of individual cells division or increase in their biomass

the rate of cell death

[#]

11.

The height to diameter ratio (H/D) for the tank fermenters is

<1.5

>1.5

[#]

12.

A reactor has a total volume of 50,000 litres. If it has a headspace volume of 10,000 litres, then volume of liquid in the reactor will be

10,000 l

20,000 l

30,000 l

40,000 l

[#]

13.

If the stirrer speed is too slow, bubbles will accumulate and coalescese under the impeller. This phenomenon is known as a

coalesced impeller

flooded impeller

river impeller

dry impeller

[#]

14.

In a two-phase system where the continuous phase remains in place, the gas hold up can be expressed as (where V_s and V_t are the superficial gas velocity and bubble rise velocity)

H = V_s/(V_s-V_t)

H = V_s/(V_s+ V_t)

H = V_s/(V_s.V_t)

H = (V_s+V_t)/V_s

[#]

15.

The approach which is commonly used to determine the level of damage to animal cells in a culture medium is to monitor

the concentration of lactate in the medium

the level of lactate oxidase activity in the medium

the level of lactate dehydrogenase activity

the level of lactase activity in the medium

[#]

16.

Which of the following affect the Kolmogorov eddy size?

Stirrer speed

Physical properties of the liquid

Size and type of impeller

All of these

[#]

17.

Model proposed by the Friedlander, correlating the air filtration efficiency by impaction can be expressed as (where N_St is the Stokes number)

η_imp = 0.075N_St^1.2

η_imp= 0.75N_St^1.2

η_imp = 0.25N_St^1.2

η_imp = 0.025N_St^1.2

[#]

18.

The collection efficiency by impaction increases with

increasing particle diameter

increasing air flow velocity

both (a) and (b)

increasing particle size

[#]

19.

The optimum wet solid content for the cell suspension for a bead mill is typically somewhere between

5-15% by volume

15-30% by volume

30-60% by volume

60-90% by volume

[#]

20.

Sedimentation is a physical process used in wastewater treatment to

remove particles that are less dense than water

remove particles that are more dense than water

remove the pertinacious material from the water

none of the above

[#]

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Online Biochemical Engineering Test - Biochemical Engineering Test 4

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