Biochemistry - Protein Purification - Discussion

Discussion Forum : Protein Purification - Section 1 (Q.No. 6)
A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
1,000 units/mg
10,000 units/mg.
100,000 units/mg
1,000,000 units/mg
Answer: Option
No answer description is available. Let's discuss.
4 comments Page 1 of 1.

Bhushan K said:   5 years ago
I agree @Deopurkar According to definition of unit given in the problem, Specific activity should be 100 units/mg.

R L Deopurkar said:   6 years ago
1 unit has been defined in the problem as 1 millimole synthesised per second.

It clearly says 10 microgram protein has one unit activity. So Specific activity is I unit/ 10 microgram protein and therefore it is 100units/mg.

This alternative is not given. Why should we change the given definition of the unit of enzyme in the problem?

Agreed that international definition of a unit is different.

Sree said:   7 years ago
Specific activity = 1/10.

= 0.1 to mg.
= 0.1/1000.
= 0.0001 mg.

This should be working right?

Ambiha said:   9 years ago
Specific activity = enzyme in sec/ total protein; units micro moles per sec/mg.

Hence conversion of unit = 1000 micro mole/10^(-2).

= 100,000.

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