Biochemistry - Immunological Techniques - Discussion

Discussion Forum : Immunological Techniques - Section 1 (Q.No. 17)

Immunological Techniques

17.

What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would not bind and be washed away

The OD values would be nearly the same as the assay control

Both (a) and (b)

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

Answer: Option

Explanation:

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