Biochemistry - Immunological Techniques

Exercise :: Immunological Techniques - Section 1

  • Immunological Techniques - Section 1
16. 

DNA- footprinting is a suitable technique for identifying which of the following?

A. Particular mRNA in mixture
B. Particular t-RNA in mixture
C. Introns within DNA
D. Protein binding site within DNA

Answer: Option D

Explanation:

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17. 

What would happen if serum is omitted from the ELISA, but all other steps remain same?

A. Anti-human Ig-conjugate would not bind and be washed away
B. The OD values would be nearly the same as the assay control
C. Both (a) and (b)
D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

Answer: Option C

Explanation:

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18. 

RAST test (radio allergosorbent test) is often used to detect

A. antibodies associated with allergies (IgE)
B. antigen associated with allergies (IgE)
C. bacteriophages
D. None of the above

Answer: Option A

Explanation:

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19. 

What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?

A. The ELISA would not develop when the substrate was added
B. The ELISA would develop normally
C. All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
D. None of the above

Answer: Option C

Explanation:

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20. 

An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

A. there was more than one antigen
B. the antibody was impure
C. the temperature was too high
D. there was one antibody and one antigen

Answer: Option A

Explanation:

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