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Biochemistry - Immunological Techniques

Exercise : Immunological Techniques - Section 1
  • Immunological Techniques - Section 1
16.
DNA- footprinting is a suitable technique for identifying which of the following?
Particular mRNA in mixture
Particular t-RNA in mixture
Introns within DNA
Protein binding site within DNA
Answer: Option
Explanation:
No answer description is available. Let's discuss.
17.
What would happen if serum is omitted from the ELISA, but all other steps remain same?
Anti-human Ig-conjugate would not bind and be washed away
The OD values would be nearly the same as the assay control
Both (a) and (b)
Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Answer: Option
Explanation:
No answer description is available. Let's discuss.
18.
RAST test (radio allergosorbent test) is often used to detect
antibodies associated with allergies (IgE)
antigen associated with allergies (IgE)
bacteriophages
None of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
19.
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added?
The ELISA would not develop when the substrate was added
The ELISA would develop normally
All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate
None of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
20.
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that
there was more than one antigen
the antibody was impure
the temperature was too high
there was one antibody and one antigen
Answer: Option
Explanation:
No answer description is available. Let's discuss.
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