Biochemistry - HPLC - Discussion
Discussion Forum : HPLC - Section 1 (Q.No. 8)
8.
Which of the following statements is true for a refractive index detector in HPLC?
Discussion:
5 comments Page 1 of 1.
Kalairajan said:
6 years ago
We can't run gradient profile in RID, it is a universal detector so we can use any kind of solute and macro-molecule.
Venkata Ravichandra Reddy said:
8 years ago
Answer B also correct, we can't run in gradient mode with RID, can be run in iscratic mode only.
Nuansri Niwattisaiwong said:
9 years ago
The answer (C) is wrong. The correct one is B.
Refractive index is a physical constant which many compounds have NEARLY the same value.(similar to melting point) = not specific, respond to many compounds. C - WRONG.
If the mobile phase come along with very small amount of the analyte, no change in the signal (refractive index of the mobile phase is still the same) = not sensitive. A - WRONG.
But if the composition of mobile phase is changed (gradient), large amount (compare to the analyte) of quantity in the composition is change, then the signal (refractive index) will change due to the change in composition of mobile phase. This huge change will overlay the signal due to the analyte which is much less than mobile phase. B - CORRECT.
Refractive index is a physical constant which many compounds have NEARLY the same value.(similar to melting point) = not specific, respond to many compounds. C - WRONG.
If the mobile phase come along with very small amount of the analyte, no change in the signal (refractive index of the mobile phase is still the same) = not sensitive. A - WRONG.
But if the composition of mobile phase is changed (gradient), large amount (compare to the analyte) of quantity in the composition is change, then the signal (refractive index) will change due to the change in composition of mobile phase. This huge change will overlay the signal due to the analyte which is much less than mobile phase. B - CORRECT.
A M K SWAMY said:
9 years ago
Answer is wrong both A and B are correct.
Tekram sahu said:
1 decade ago
RI DETECTOR USED ONLY FOR CARBOHYDRATES LIKE GLUCOSE, FOS (FRUCTO OLIGOSACHHARIDES) OR FRUCTOSE, SUCROSS, MALTOSE etc.
It is universal detector. And specific. Its depend upon refraction of the molecule they path phage is change one medium to another.
It is universal detector. And specific. Its depend upon refraction of the molecule they path phage is change one medium to another.
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