Biochemistry - HPLC

Exercise :: HPLC - Section 1

  • HPLC - Section 1
6. 

An isocratic elution in HPLC is one in which the composition of the solvent

A. remains constant
B. changes continuously
C. changes in a series of steps
D. none of these

Answer: Option A

Explanation:

No answer description available for this question. Let us discuss.

7. 

In reversed phase HPLC, there is a

A. non polar solvent/polar column
B. polar solvent/non-polar column
C. non polar solvent/non-polar column
D. any of the above

Answer: Option B

Explanation:

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8. 

Which of the following statements is true for a refractive index detector in HPLC?

A. It is more sensitive than a UV detector
B. It can only be used for isocratic elutions
C. It does not respond to many solutes
D. none of above

Answer: Option C

Explanation:

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9. 

A gradient elution in HPLC is one in which the composition of the solvent

A. remains constant
B. is changed continuously or in a series of steps
C. both (a) and (b)
D. none of the above

Answer: Option B

Explanation:

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10. 

An eluotropic series

A. ranks solvents by their relative abilities to displace solutes from a given absorbent
B. ranks column packing material by their relative abilities to retain solutes on the column
C. is a measure of the solvent adsorption energy
D. none of the above

Answer: Option A

Explanation:

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