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Biochemistry - HPLC

Exercise : HPLC - Section 1
  • HPLC - Section 1
11.
Dwell volume is defined as
the volume of solvent contained in a liquid chromatographic column
the time required for the gradient to reach the column
the volume of the column between the point at which solvents are mixed and the beginning of the column
none of these
Answer: Option
Explanation:
No answer description is available. Let's discuss.
12.
Void volume refers to the
total volume of eluent in the column the remainder being taken up by the packing material
the volume of solvent contained in a liquid chromatographic column
the time required for the gradient to reach the column
the volume of the column between the point at which solvents are mixed and the beginning of the column
Answer: Option
Explanation:
No answer description is available. Let's discuss.
13.
In normal phase HPLC, there is a
non polar solvent/polar column
polar solvent/non-polar column
non polar solvent/non-polar column
any of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
14.
Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?
Greater sensitivity and reusable columns
Ideal for ionic species and large molecules
Sample recovery
All of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
15.
Which can be used as a mobile phase in HPLC applications?
Any compound with solubility in liquid
Any compound with limited solubility in liquid
Any compound with non-solubility in liquid
Any of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
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