Biochemistry - HPLC

Exercise :: HPLC - Section 1

  • HPLC - Section 1
11. 

Dwell volume is defined as

A. the volume of solvent contained in a liquid chromatographic column
B. the time required for the gradient to reach the column
C. the volume of the column between the point at which solvents are mixed and the beginning of the column
D. none of these

Answer: Option C

Explanation:

No answer description available for this question. Let us discuss.

12. 

Void volume refers to the

A. total volume of eluent in the column the remainder being taken up by the packing material
B. the volume of solvent contained in a liquid chromatographic column
C. the time required for the gradient to reach the column
D. the volume of the column between the point at which solvents are mixed and the beginning of the column

Answer: Option A

Explanation:

No answer description available for this question. Let us discuss.

13. 

In normal phase HPLC, there is a

A. non polar solvent/polar column
B. polar solvent/non-polar column
C. non polar solvent/non-polar column
D. any of the above

Answer: Option A

Explanation:

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14. 

Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)?

A. Greater sensitivity and reusable columns
B. Ideal for ionic species and large molecules
C. Sample recovery
D. All of the above

Answer: Option D

Explanation:

No answer description available for this question. Let us discuss.

15. 

Which can be used as a mobile phase in HPLC applications?

A. Any compound with solubility in liquid
B. Any compound with limited solubility in liquid
C. Any compound with non-solubility in liquid
D. Any of the above

Answer: Option A

Explanation:

No answer description available for this question. Let us discuss.

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