Online Biochemical Engineering Test - Biochemical Engineering Test - Random

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Total number of questions: 20.
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Given an enzyme with a K_m = 10m M and V_max = 100 m mol/min. If [S] = 100 m M, which of the following will be true?

A 10 fold increase in V_max would increase velocity 10 fold y

A 10 fold decrease in K_m would increase velocity

Both (a) and (b)

A 10 fold increase in V_max would decrease velocity 20 fold

[#]

Most enzymes work by

increasing energy of activation

decreasing energy of activation

not affecting energy of activation

none of the above

[#]

Lock and key theory is based on the compatibility of

enzyme and substrate

enzyme and product

enzyme and enzyme substrate complex

enzyme substrate complex and product

[#]

Classical noncompetitive inhibition is obtained only under

slow equilibrium conditions

moderate equilibrium conditions

rapid equilibrium conditions

non-equilibrium conditions

[#]

Which of the following is considered as a disadvantage to the adsorption method of immobilization?

It is possible to separate and purify the enzymes while being immobilized

The enzymes are not usually deactivated by adsorption

The adsorption is a reversible process

State of immobilization is very sensitive to solution pH, ionic strength and temperature

[#]

To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as

reaction rate/rate if not slowed by diffusion

rate if not slowed by diffusion/ reaction rate

actual reaction rate/ rate if not slowed by diffusion

rate if not slowed by diffusion/ actual reaction rate

[#]

Lysozyme

catalyses hydrolysis of β-1-4 linkages between N-acetyl muranic acid and N-acetyl glucosamine in peptideoglycan

catalyses hydrolysis of α-1-4 linkages between N-acetyl muranic acid and N-acetyl glucosamine in peptideoglycan

catalyses hydrolysis of α-1-4 linkages between N-diacetyl muranic acid and N-diacetyl glucosamine in peptideoglycan

all of the above

[#]

Fluidized bed bioreactors provide higher mass transfer rates than packed bed bioreactors because

mixing is higher in fluidized bed bioreactors

particles move with the fluid in a fluidized bed bioreactor

immobilized particles are smaller in the fluidized bed bioreactors

all of the above

[#]

A strain of Escherichia coli has a maximum specific growth rate of 0.8 h^-1 on a glucose based medium. If this organism is being grown in a chemostat with a dilution rate of 1.2 h^-1, then at steady state the concentration of E. coli in the same medium will

increase

be zero

change randomly

decrease

[#]

10.

In the death phase

nutrients available for the cells are depleted and begin to die

the number of viable cells will increase

nutrients available for cells are replenished and start to multiply

none of the above

[#]

11.

What is the ratio of diameter of impeller to the diameter of tank (D_a:D_t)?

1 : 3

2 : 1

4 : 1

1 : 5

[#]

12.

Impeller damage can be minimized by

using specially designed axial flow impellers

using radial flow impellers

using Intermig impellers

all of the above

[#]

13.

During fermentation, the saturation concentration of oxygen was reduced from 7 ppm. to 1 ppm. Which one of the following conclusions can be drawn?

Microbial growth is oxygen limited since the oxygen concentration is lesser than the saturation concentration

Microbial growth is not oxygen limited since the oxygen concentration is lesser than the saturation concentration

Experimental error leads to the reduction

No conclusion can be drawn

[#]

14.

A pseudoplastic fluid is one in which the viscosity

is constant regardless of the stirrer speed or mixing time

changes during mixing but returns to its original state after mixing

increases with increasing stirrer speed

decreases with increasing stirrer speed

[#]

15.

The collection efficiency by the inertial impaction mechanism is the function of

Stokes and Reynolds number

Stokes and Schmidt number

Grashoff and Reynolds number

Stokes and Grashof number

[#]

16.

The K used in the equation for X₁₀=2.303/K depends upon

nature of the filter material

linear velocity of the air passing through filter

both (a) and (b)

thickness of the filter

[#]

17.

For cost analysis in a fermentor system, the depreciation cost varies from

5-6% of the capital cost

6-7% of the capital cost

6-10% of the capital cost

8-10% of the capital cost

[#]

18.

Start up expenses in a fermentation industry varies from

0-5% of the capital cost

10-15% of the capital cost

5-10% of the capital cost

15-20% of the capital cost

[#]

19.

Hardness of water does not

have any bad effect in boiler

make cooking of foods difficult

make it unfit for drinking

cause difficulty in the washing of clothes with soaps

[#]

20.

The activated sludge process consists of returning a portion of the clarifier

effluent water entering the reactor

influent water coming out of the reactor

influent water entering the reactor

effluent water coming out of the reactor

[#]

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