Online Biochemical Engineering Test - Biochemical Engineering Test 7

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Total number of questions: 20.
Time allotted: 30 minutes.
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For an enzyme that displays Michaelis-Menten kinetics, the reaction velocity (as a fraction of V_max) observed at [S] = 2 K_M will be

0.09

0.33

0.66

0.91

[#]

Which of the following step is assumed to be the slowest step in the Michaelis Menten equation?

The substrate consuming step

The product releasing step

Formation of enzyme substrate complex

None of these

[#]

A classical noncompetitive inhibitor has

no effect on substrate binding

no effect on substrate binding and vice versa

significant effect on substrate binding

significant effect on substrate binding and vice versa

[#]

Predominantly uncompetitive inhibition may be called when

competitive inhibition is greater than uncompetitive inhibition

competitive inhibition is smaller than uncompetitive inhibition

competitive inhibition is equal to uncompetitive inhibition

none of the above

[#]

The lowest biomass yield in a culture of Escherichia coli will be in

an aerated batch culture containing a initial high concentration of glucose

an aerated batch reactor containing an initial low concentration of glucose

an aerated fed-batch reactor having a low glucose concentration

an aerated continuous reactor having a low glucose concentration

[#]

Power number is ratio of

imposed forced to intertial force

bouyant force to inertial force

gravitation force to inertial force

imposed force to gravitational force

[#]

Power number (N_p) is also known as

Froude's number

Bond's number

Newton's number

Rayleigh's number

[#]

A fermentation system has a K_La of 3 s^-1 and a C₀* of 5 ppm of O₂. If the bulk liquid is completely depleted of oxygen, then the oxygen transfer rate will be equal to

zero

15 mg.l^-1.s^-1

10 mg.l^-1.s^-1

5 mg.l^-1.s^-1

[#]

The power required by an agitator operating in a gas-sparged system is

lesser than the power required by an impeller in operating at same speed in a gas free system

more than the power required by an impeller in operating at same speed in a gas free system

same as the power required by an impeller in operating at same speed in a gas free system

none of the above

[#]

10.

Axial flow impellers

provide high shear conditions for breaking up bubbles

provide low shear conditions and are suited for use in shear sensitive procesess

provide direct flow towards the walls of the reactor

none of the above

[#]

11.

In a bioreactor, if at a particular time the oxygen transfer rate is 23 kg.h^-1, then the oxygen uptake will be

23 kg.h^-1

less than 23 kg.h^-1

greater than 23 kg.h^-1

2.3 kg.h^-1

[#]

12.

Bubble free aeration systems requires

silicone tubing or gas permeable membranes to facilitate gas transfer

carbon dioxide instead of air to supply oxygen

silicone tubing or gas permeable membranes to remove carbon dioxide by oxygen in the form of hydrogen peroxide

filled headspace of the reactor with carbon dioxide

[#]

13.

If the viscosity of a fluid decreases with increasing stirrer speed, then the nature of the fluid is

newtonian

pseudoplastic

dilatant

thixotropic

[#]

14.

Water is a

newtonian fluid

pseudoplastic

dilatant

rheopectic

[#]

15.

The relationship between the del factor, temperature and time is given as

Δ = A.t. e^-E/RT

Δ = 1/(A.t. e^-E/RT)

Δ = A.t. e^E/RT

Δ = A.t.T

[#]

16.

Which of the following is considered as a pretreatment to the biological feeds?

Heating to denature the proteins

Addition of filter aids to increase the porosity

Addition of electrolytes

All of the above

[#]

17.

Conventional adsorption is a

reversible process

irreversible process

either reversible or irreversible

none of these

[#]

18.

Which of the operation does not come under upstream processing?

Media preparation

Inoculum development

Effluent treatment

Storage of raw material

[#]

19.

Sanitizers used for rubber made equipments are

strong acids

strong alkalis

combination of both

none of these

[#]

20.

The common methods used for disinfection in waste water treatment plants are

chlorination

UV light

both (a) and (b)

Phenolic solvent

[#]

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Online Biochemical Engineering Test - Biochemical Engineering Test 7

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