Microbiology - DNA Replication - Discussion

Discussion :: DNA Replication - Section 1 (Q.No.2)


Proofreading activity to maintain the fidelity of DNA synthesis

[A]. occurs after the synthesis has been completed
[B]. is a function of the 3'-5' exonuclease activity of the DNA polymerases
[C]. requires the presence of an enzyme separate from the DNA polymerases
[D]. occurs in prokaryotes but not eukaryotes

Answer: Option B


No answer description available for this question.

Renuka said: (Apr 30, 2013)  
Proofreading activity to maintain the fidelity of DNA synthesis? How they Work?

Mitrabinda said: (Jul 5, 2013)  
The function of DNA polymerase is to add new nucleotides to the elongation chain, by that time there is a chances of incorporation of wrong base pairs, so the proofreading activity of the enzyme ensures the removal of such wrong bases in both directions & adding correct bases. This occurs by enzymes 3'-5' & 5'-3' exonuclease activity.

Noursein said: (Feb 28, 2016)  
I actually didn't understand this question and its answer I urgently need the explanation of this question because I have a biology exam tomorrow.

Nanoshe Taye said: (May 1, 2017)  
Proofreading exonuclease is capable of degrading DNA starting from 3'DNA end, that is from the growing end of the new DNA.

Timothy said: (Nov 12, 2018)  
Once all bases are matched up (A with T, C with G) an enzyme called the exonuclease strips away the primers. The gaps were the primers were are then filled by yet more complementary nucleotides. The new strand is now proofread by DNA polymerase to make sure there is no mistakes in the DNA sequence.

Nishtha Goyal said: (May 7, 2019)  
It means that this process occurs after the formation of the replication fork.

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