Biotechnology - Recombinant DNA - Discussion
Discussion Forum : Recombinant DNA - Section 1 (Q.No. 3)
3.
Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using
Discussion:
8 comments Page 1 of 1.
Setina said:
3 weeks ago
Expression vectors are a type of plasmid or virus specifically designed for gene expression in a host cell.
While other vectors like Bacterial Artificial Chromosomes (BACs) and Yeast Artificial Chromosomes (YACs) are used for cloning very large DNA fragments, they aren't optimised for producing large quantities of protein.
While other vectors like Bacterial Artificial Chromosomes (BACs) and Yeast Artificial Chromosomes (YACs) are used for cloning very large DNA fragments, they aren't optimised for producing large quantities of protein.
Ayush Mittal said:
12 months ago
BAC and YAC are suitable for inserting GOI with large sizes but cannot produce protein as they are not expression vectors.
Raj said:
6 years ago
Yes, but good vectors for cloning large DNA. But expression vectors are specific for the expression for an insered gene.
Vinita said:
8 years ago
Bac and yac are advance form of cloning vectors, they are not expression vectors.
(1)
Deep said:
8 years ago
Why option D is not correct? As BAC and YAC are also expression vector's.
Afzal said:
8 years ago
Vector is not just a plasmid. There are many types of vectors. Like bacterial artificial chromosome and Yeast chromosome etc.
Caroline said:
10 years ago
Hi, according to wiki, An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells.
The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene.
The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene.
ANJU said:
1 decade ago
Recombinant DNA works when the host cell expresses protein from the recombinant genes.
A significant amount of recombinant protein will not be produced by the host unless expression Factors are added.
A significant amount of recombinant protein will not be produced by the host unless expression Factors are added.
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