Biotechnology - Recombinant DNA - Discussion
Discussion Forum : Recombinant DNA - Section 1 (Q.No. 8)
8.
Which of the following is obtained using processed mRNA molecules as a template?
Discussion:
2 comments Page 1 of 1.
Priyanka said:
7 years ago
mRNA is used as the very first step in the creation of cDNA library. MRNA is first acted upon by Reverse transcriptase and this ultimately leads to the formation of cDNA library.
Ritu said:
1 decade ago
cDNA is a complementary DNA of processed m RNA.
In eukaryotes the gene is interrupted with introns (non coding regions) to obtain the desire target gene cDNA is used for further processing. That's why these processed m RNA (without introns) is used from which we make cDNA.
In eukaryotes the gene is interrupted with introns (non coding regions) to obtain the desire target gene cDNA is used for further processing. That's why these processed m RNA (without introns) is used from which we make cDNA.
(1)
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