Biotechnology - Recombinant DNA - Discussion

Discussion :: Recombinant DNA - Section 1 (Q.No.23)


The advantage of using DNA polymerases from thermophilic organisms in PCR is that

[A]. the DNA polymerases of these bacteria are much faster than those from other organisms
[B]. the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
[C]. the DNA polymerases of these bacteria never make mistakes while replicating DNA
[D]. all of the above

Answer: Option B


No answer description available for this question.

Samuel Terkper Ahuno said: (Sep 27, 2015)  
Thermophilic as the name suggests, are -heat loving organisms. They have the ability to withstand high conditions of temperature. DNA pols from these organisms are widely used.

Anu said: (Nov 15, 2016)  
Denaturation of DNA means separating two strands of DNA by using some bacterias at temp 94 to 95-degree celsius.

Noumal Hassni said: (Jul 16, 2020)  
The polymerase chain reaction is a thermo-cycling process involving high temperature as 94°C, DNA polymerase of thermophilic bacteria can survive such temperature. An example is a commonly used Taq-polymerase enzyme isolated from Thermophylus aquatics.

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