Biotechnology - Recombinant DNA - Discussion
Discussion Forum : Recombinant DNA - Section 1 (Q.No. 20)
20.
Which type of restriction endonuclease cuts the DNA within the recognition site?
Discussion:
3 comments Page 1 of 1.
Srejan said:
7 years ago
Type I enzymes cleave at sites remote from a recognition site.
Type II enzymes cleave within or at short specific distances from a recognition site. The most common Type II enzymes are HhaI, HindIII, and NotI, that cleave DNA within their recognition sequences.
Type III enzymes cleave at sites a short distance from a recognition site.
Type IV enzymes target modified DNA, Eg. Methylated, hydroxymethylated and glucosyl hydroxymethylated DNA.
Type II enzymes cleave within or at short specific distances from a recognition site. The most common Type II enzymes are HhaI, HindIII, and NotI, that cleave DNA within their recognition sequences.
Type III enzymes cleave at sites a short distance from a recognition site.
Type IV enzymes target modified DNA, Eg. Methylated, hydroxymethylated and glucosyl hydroxymethylated DNA.
Sumitha said:
9 years ago
Type I enzyme - Cut DNA at random far from their recognition sequences.
Type II enzyme - Cut DNA at defined positions close to or within their recognition sequences.
Type III enzyme - Cleave outside of their recognition sequences.
Type II enzyme - Cut DNA at defined positions close to or within their recognition sequences.
Type III enzyme - Cleave outside of their recognition sequences.
Leesarani Sahani said:
9 years ago
What are the types of Types ll endonuclease and why another type of endonuclease can not cut that recognition site?
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