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Biochemistry - Recombinant DNA Technology - Discussion

Discussion Forum : Recombinant DNA Technology - Section 1 (Q.No. 18)
Recombinant DNA Technology
18.
The order for the construction of a cDNA fragment from mRNA is to
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
Answer: Option
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