Biochemistry - Polymerase Chain Reaction - Discussion

Discussion Forum : Polymerase Chain Reaction - Section 1 (Q.No. 15)
15.
How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?
Two ds DNA molecule
Three ds DNA molecule
Eight ds DNA molecule
Four ds DNA molecule
Answer: Option
Explanation:
No answer description is available. Let's discuss.
Discussion:
10 comments Page 1 of 1.

Amartey Clinton said:   3 years ago
It should be 16, since the number of DNA molecules produced at the end of each cycle is =2^n.

Where n is the number of cycles.

So =>2^4 = 16.

Alisha said:   4 years ago
I think it should be 16.

Rashed said:   6 years ago
It should be 16.

Marshy said:   7 years ago
If the question is DNA molecule after the completion 4th cycle then it will not b 4. It is 16.

The formulae is 2 to the power n. N is number of cycle.

EMANUEL said:   7 years ago
The answer should be Two ds DNA molecule.

Linshi said:   7 years ago
You are correct @Devanshi.

Devanshi said:   8 years ago
Answer is correct because in question, you can see they ask during 4th cycle not after completing 4th cycle. So the Answer would be 4.

Priyanka said:   8 years ago
I also agree with you @Ann.

The given answer of 4 ds DNA is wrong.

Ann said:   9 years ago
1 cycle of PCR yields 2 ds DNA, then obviously 4 cycles should give 8 ds DNA.

Abdullah said:   1 decade ago
How this possible? The question is unclear. How many DNA molecule are present before 4th cycle?

If 1 then the answer is 2 and it's right but if we start from first and go step wise then the answer is 8.

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