Biochemistry - Nucleic Acids - Discussion
Discussion Forum : Nucleic Acids - Section 1 (Q.No. 1)
1.
Because DNA is a highly charged polyanion, its stability to heat denaturation
Discussion:
7 comments Page 1 of 1.
Muhas student said:
6 years ago
Since most salts are hydrophilic, addition of salts makes the salts constricted and being stacked on one another hence caising an increase in Tm of DNA molecules.
(1)
Vivian said:
7 years ago
Thanks @Vinayak.
Vinayak said:
1 decade ago
Increase in salt concentration mask the charge on both strands of DNA. Thus making them very stable and the resultant repulsion force will be minimized. As a result the stability of DNA increases with increase in salt concentration and require higher temperature than in absence of salt.
Faisal biochemist said:
1 decade ago
As high salt concentrations mask the destabilising charge repulsion between the two negatively charged phosphodiester backbones. DNA duplex stability therefore increases with increasing salt concentration.
COOKEY said:
1 decade ago
Yeah that's absolutely true, acidic salts like NaHSO4.
Bernadette said:
1 decade ago
Acidic salts increase the stability of DNA because they are made of bases purines and pyrimidines.
Deus Mselle said:
1 decade ago
How does the increase in salt concentration increase the stability and which types of salts?
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