Biochemistry - Nucleic Acids - Discussion

Discussion :: Nucleic Acids - Section 1 (Q.No.1)


Because DNA is a highly charged polyanion, its stability to heat denaturation

[A]. does not depend on hydrophobic interactions
[B]. increases with increasing salt
[C]. is independent of G - C content
[D]. decreases with increasing salt

Answer: Option B


No answer description available for this question.

Deus Mselle said: (Nov 8, 2012)  
How does the increase in salt concentration increase the stability and which types of salts?

Bernadette said: (Jan 9, 2013)  
Acidic salts increase the stability of DNA because they are made of bases purines and pyrimidines.

Cookey said: (Nov 26, 2013)  
Yeah that's absolutely true, acidic salts like NaHSO4.

Faisal Biochemist said: (Jun 7, 2014)  
As high salt concentrations mask the destabilising charge repulsion between the two negatively charged phosphodiester backbones. DNA duplex stability therefore increases with increasing salt concentration.

Vinayak said: (Aug 19, 2014)  
Increase in salt concentration mask the charge on both strands of DNA. Thus making them very stable and the resultant repulsion force will be minimized. As a result the stability of DNA increases with increase in salt concentration and require higher temperature than in absence of salt.

Vivian said: (Dec 22, 2018)  
Thanks @Vinayak.

Muhas Student said: (Jun 5, 2019)  
Since most salts are hydrophilic, addition of salts makes the salts constricted and being stacked on one another hence caising an increase in Tm of DNA molecules.

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