Biochemistry - Genetic Code and Regulation - Discussion

Discussion Forum : Genetic Code and Regulation - Section 1 (Q.No. 1)
1.
Which of the following techniques was carried out by Nirenberg and Matthaei in 1961 to determine the first codon?
In vitro synthesis of a polypeptide using UUUUU
Labeled peptide binding to a ribosome
Mixed co-polymer mRNA synthesis
none of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
Discussion:
1 comments Page 1 of 1.

Shweta said:   1 decade ago
This was the easiest methods to find which codon codes for which Amino acid. If a sequence is generated such that it contains all Uracils or Thymines, e.g UUUU, irrespective of what the reading frame only 1 type of Amino acid will be coded and polymerised.

In the case of UUUU. It ll be a peptide having repeated units of phenylalanine as UUU codes for it. Analysing such a peptide is easy. Further variations in the repeated units led to the discovery of all codons.

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