Biochemistry - Gel Electrophoresis - Discussion
Discussion Forum : Gel Electrophoresis - Section 1 (Q.No. 5)
5.
In isoelectric focusing, proteins are separated on the basis of their
Discussion:
1 comments Page 1 of 1.
Gopal agrawal said:
1 decade ago
Isoelectric focusing means the separation of the protein based on the isoelectric pH. Isoelectric pH is the pH at which the protein can exist at the neutral state.
For being neutral it should have both positive and negative charged residue. Thus isoelectric focusing is based on the relative content of positively and negatively charged residue.
For being neutral it should have both positive and negative charged residue. Thus isoelectric focusing is based on the relative content of positively and negatively charged residue.
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