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Biochemistry - Gel Electrophoresis - Discussion

Discussion Forum : Gel Electrophoresis - Section 1 (Q.No. 4)
Gel Electrophoresis
4.
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in
both proteins migrate to the anode
histones migrate to the anode and myoglobin migrates to the cathode
histones migrate to the cathode and myoglobin migrates to the anode
both proteins migrate to the cathode
Answer: Option
Explanation:
No answer description is available. Let's discuss.
Discussion:
3 comments Page 1 of 1.
Sirisha said:   7 years ago
As compared to PI, Increased pH leads increased the H+ ion concentration, so it migrates towards -vely charged electrode (Cathode). When in decreased pH, increases the OH- ion concentrations, then it migrates towards +vely charged electrode (Anode).
Tehale Hailu said:   1 decade ago
Cathode is negative while anode is positive, so positively charged amino acid migrated towards this and negatively charged amino acid migrates towards the anode.
Shweta said:   1 decade ago
Histones have a pI of 8. 5 while that of myoglobin is 5. 5. At pH 7 the histones have a positive charge while the myoglobin has negative charge.
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