Biochemistry - Gel Electrophoresis - Discussion

Discussion :: Gel Electrophoresis - Section 1 (Q.No.4)

4. 

Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in

[A]. both proteins migrate to the anode
[B]. histones migrate to the anode and myoglobin migrates to the cathode
[C]. histones migrate to the cathode and myoglobin migrates to the anode
[D]. both proteins migrate to the cathode

Answer: Option C

Explanation:

No answer description available for this question.

Shweta said: (Apr 14, 2012)  
Histones have a pI of 8. 5 while that of myoglobin is 5. 5. At pH 7 the histones have a positive charge while the myoglobin has negative charge.

Tehale Hailu said: (Apr 29, 2014)  
Cathode is negative while anode is positive, so positively charged amino acid migrated towards this and negatively charged amino acid migrates towards the anode.

Sirisha said: (Jan 16, 2019)  
As compared to PI, Increased pH leads increased the H+ ion concentration, so it migrates towards -vely charged electrode (Cathode). When in decreased pH, increases the OH- ion concentrations, then it migrates towards +vely charged electrode (Anode).

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