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Biochemistry - UV Luminance Spectroscopy

Exercise : UV Luminance Spectroscopy - Section 1
  • UV Luminance Spectroscopy - Section 1
1.
What is the relationship between wavelength and wave number?
Wavenumber = 1 / wavelength in centimeters
Wavenumber - wavelength in nanometers = 1
Wavelength in nanometers x wavenumber = 1
None of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
2.
In the intersystem crossing
the spin of an excited electron reverses, changing the state of the molecule (from singlet state to triplet state or vice versa)
a molecule converts its excess energy to light, and emits a photon
a molecule converts excess electronic energy to vibrational energy
all of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
3.
Why must the radiation source for fluorescence spectrometry be more powerful than for absorption spectroscopy?
Because the magnitude of the output signal is proportional to the power of the incident radiation
Because the sample won't fluoresce if the incident radiation is of low power
To allow for scattering by the sample
None of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
4.
Why do fluorescence spectrometers often use double-beam optics?
So a reference solution can be used
To compensate for beam attenuation by the monochromator
To compensate for power fluctuations in the radiation source
All of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
5.
For a molecule to absorb IR, why must the molecule's vibrations cause fluctuations in the dipole moment of the molecule?
Because a change in dipole moment lowers the energy required for electronic transitions
Because for absorption to occur, the radiation must interact with the electric field caused by changing dipole moment
Because fluctuations in the dipole moment allow the molecule to deform by bending and stretching
All of the above
Answer: Option
Explanation:
No answer description is available. Let's discuss.
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