Biochemistry - Recombinant DNA Technology

Exercise :: Recombinant DNA Technology - Section 1

  • Recombinant DNA Technology - Section 1
21. 

Which type of restriction enzymes do not usually require ATP?

A. Type I
B. Type II
C. Type III
D. Type IV

Answer: Option B

Explanation:

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22. 

An example of a restriction fragment length polymorphism is

A. an Eco RI cuts DNA at a different sequence than Hind III
B. different length fragments of DNA resulting from loss or gain of a restriction site
C. cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
D. all of the above

Answer: Option B

Explanation:

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23. 

Knockout mice are created by

A. mutagenizing a mouse and selecting for mutant offspring
B. creating a chimera by fusing cells from two different cell lines
C. infecting the mouse with a retrovirus
D. transfecting embryonic stem cells with an altered gene sequence

Answer: Option D

Explanation:

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24. 

A plasmid

A. is a circular DNA molecule
B. always contains an origin of replication
C. usually contains one or more restriction sites
D. all of the above

Answer: Option D

Explanation:

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25. 

Under which of the following conditions would population gene frequencies remain the same?

A. Selection for homozygotes
B. Small population size
C. Active migration between groups
D. Random mating

Answer: Option D

Explanation:

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