Biochemistry - Recombinant DNA Technology

Exercise :: Recombinant DNA Technology - Section 1

  • Recombinant DNA Technology - Section 1
11. 

Which of the following techniques can be used to determine the defective gene and for developing cancer?

A. Western blot
B. Southern blot
C. Northern blot
D. Eastern blot

Answer: Option B

Explanation:

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12. 

The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .

A. would be of no concern if it occurred.
B. has occurred
C. can never occur
D. seems unlikely

Answer: Option D

Explanation:

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13. 

Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

A. in both cases molecules migrate to the anode
B. both techniques rely on a constant charge to mass ratio
C. both techniques utilize the sieving properties of gels
D. all of the above

Answer: Option D

Explanation:

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14. 

Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

A. copies of the gene with an altered sequence so that a restriction site is inserted
B. mutagenized copies of a gene
C. short sequences that will hybridize only to a specific base sequence
D. PCR-amplified variable numbers of tandem repeats (VNTRs)

Answer: Option C

Explanation:

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15. 

In order to insert a foreign gene into a plasmid, both must __________

A. have identical DNA sequences
B. originate from the same type of cell
C. be cut by the same restriction enzyme
D. be of the same length

Answer: Option C

Explanation:

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