The fact that β-oxidation of fatty acids, occurs in the mitochondria whereas fatty acid synthesis occurs in the cytoplasmic matrix, is an example of regulation of enzymatic activity by the __________ mechanism.
The affinity of an enzyme for its substrate, when the enzyme has a Km of 0.50 M will be __________ than the affinity of an enzyme for its substrate when the enzyme has a Km of 0.05 M.