How does the increase in salt concentration increase the stability and which types of salts?
(Wed, Jan 9, 2013 02:34:47 PM)
Acidic salts increase the stability of DNA because they are made of bases purines and pyrimidines.
(Tue, Nov 26, 2013 08:03:13 PM)
Yeah that's absolutely true, acidic salts like NaHSO4.
Faisal Biochemist said:
(Sat, Jun 7, 2014 05:24:45 PM)
As high salt concentrations mask the destabilising charge repulsion between the two negatively charged phosphodiester backbones. DNA duplex stability therefore increases with increasing salt concentration.
(Tue, Aug 19, 2014 03:17:38 PM)
Increase in salt concentration mask the charge on both strands of DNA. Thus making them very stable and the resultant repulsion force will be minimized. As a result the stability of DNA increases with increase in salt concentration and require higher temperature than in absence of salt.