Biochemistry - HPLC

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  • Section 1
"Act well your part; there all honor lies."
- Alexander Pope

HPLC stands for

A. High Pressure Liquid Chromatography
B. High Performance Liquid Chromatography
C. both (a) and (b)
D. Highly Placed Liquid Chromatography


The eluent strength is a measure of

A. solvent adsorption energy
B. solvent absorption energy
C. solvent diffusivity
D. solvent mixing index


HPLC methods include

A. liquid/liquid (partition) chromatography
B. liquid/solid (adsorption) chromatography
C. ion exchange and size exclusion chromatography
D. all of the above


Column efficiency is measured in terms of number of plates which is

A. inversely related to the square of the peak width
B. directly related to the square of the peak width
C. inversely related to the cube root of the peak width
D. directly related to the square of the peak width


For a typical adsorbent such as silica gel, the most popular pore diameters are

A. 10 and 50 A°
B. 60 and 100 A°
C. 100 and 150 A°
D. 150 and 200 A°

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